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Zbrafish Digest, Vol 28, Issue 3

Zbrafish Digest, Vol 28, Issue 3 - Zebrafish Forum

Zbrafish Digest, Vol 28, Issue 3 - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 09-07-2007, 07:35 PM
raj abi
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Default Zbrafish Digest, Vol 28, Issue 3



Hi Sridevi,
Depending upon whether you intend to do pair spawning or group spawning,you can use different types of breeding tanks. Plasic tanks of 2 lit capacity with a fitting trap to allow the eggs settle at the bottom will be sufficient for pair spawning where as 8 lit glass tank or fibre glass tank with suitable trap will serve the purpose for group spawning.
In both cases I would suggest you can make your traps or sieve we can say using plastic or fibre glass to fit the size of the tanks.The point here is, the eggs need to be clearly separated and settled down on the bottom of the tank to save it from being eaten up by the adults,meaning eggs can pass through the sieve while the adults should not.You can attach some plastic leaves or some soft substance to the sieve/trap as they love to lay the eggs around it!!!.............and your breeding tank is ready...

rds
Rajesh Krishnan
University of Toronto

[Only registered users see links. ] wrote:
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Today's Topics:

1. Operon Zebrafish oligo probe set ([Only registered users see links. ])
2. Request information for the Zebrafish breeding tanks (sridevi)
3. RE: Request information for the Zebrafish breeding tanks
(christian lawrence)
4. RE: Request information for the Zebrafish breeding tanks
(Eric Moore)


----------------------------------------------------------------------

Message: 1
Date: Fri, 07 Sep 2007 07:17:26 -0700
From: [Only registered users see links. ]
Subject: [Zbrafish] Operon Zebrafish oligo probe set
To: [Only registered users see links. ]
Message-ID: <1189174646.952213.98410@d55g2000hsg.googlegroups. com>
Content-Type: text/plain; charset="iso-8859-1"

Hi everyone,

Operon is planning the release of a microarray ready probe set for
printing microarrays for Zebrafish. The set includes about 22,000 70
mer probes that cover the entire genome. If you or a colleague are
interested please feel free to contact me for more information.

Thank you,

Abria

[Only registered users see links. ]
256-704-8159



------------------------------

Message: 2
Date: Fri, 7 Sep 2007 12:15:05 +0100 (BST)
From: sridevi
Subject: [Zbrafish] Request information for the Zebrafish breeding
tanks
To: [Only registered users see links. ]
Message-ID: <[Only registered users see links. ].in.yahoo.com>
Content-Type: text/plain; charset="iso-8859-1"

Dear Sir/Madam,

I sridevi from India working as a Junior Research Fellow under Dr. V.Kalarani Head, Associate Professor, Department of Biotechnology, Sri Padmavati Women's University, Tirupati, Chittoor Dist., Andhra Pradesh, India.
We are working on zebrafish. So we need the zebrafish breeding tanks. Please give me the modalities and information for purchase of zebrafish breeding tanks.

With Best Regards,

Sridevi


---------------------------------
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Message: 3
Date: Fri, 7 Sep 2007 14:11:43 -0400
From: "christian lawrence"
Subject: RE: [Zbrafish] Request information for the Zebrafish breeding
tanks
To: "'sridevi'" ,

Message-ID:
Content-Type: text/plain; charset="us-ascii"

Most suppliers (AHAB, MBI, Tecniplast, etc.) make their own version of
breeding trap. Most of these, as well as others made by laboratory supply
companies, are plastic traps that fit inside plastic tanks.

In my opinion (based upon my personal experience, data collected in
laboratory settings, and observations of the animals spawning in the wild)
the ones that work best are those that can be "tipped", providing a shallow
to deep gradient within the trap. The fish appear to preferentially spawn
in the shallow areas. If you don't believe me, try it, and pay close
attention to how the fish behave and where most of the eggs are laid.

All cages will work. But those that can be easily tipped work better.
Still my opinion. But a semi-informed one.

With this in mind, we use 2 liter polycarbonate mouse cages from nalgene or
tecniplast, fill it with fish water, and insert the following breeding trap,
tip it, and let the fish do their thing (with proper conditioning, of
course).

[Only registered users see links. ]

Using such an approach, you can probably get all supplies in India (mouse
cages, reasonable facsimile of breeding trap).

Also you can check out
[Only registered users see links. ]
[Only registered users see links. ]
[Only registered users see links. ]
[Only registered users see links. ]

Good luck,
Chris



Christian Lawrence
Brigham and Women's Hospital
Karp Family Research Laboratories 06-004B
One Blackfan Circle
Boston, Massachusetts 02115
Tel: 617.355.9041
Fax: 617.355.9064




_____

From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of sridevi
Sent: Friday, September 07, 2007 7:15 AM
To: [Only registered users see links. ]
Subject: [Zbrafish] Request information for the Zebrafish breeding tanks


Dear Sir/Madam,

I sridevi from India working as a Junior Research Fellow under Dr.
V.Kalarani Head, Associate Professor, Department of Biotechnology, Sri
Padmavati Women's University, Tirupati, Chittoor Dist., Andhra Pradesh,
India.
We are working on zebrafish. So we need the zebrafish breeding tanks. Please
give me the modalities and information for purchase of zebrafish breeding
tanks.

With Best Regards,

Sridevi


_____

5, 50, 500, 5000 - Store N number of mails in your inbox. Click
l/yahoomail/tools/tools-08.html/> here.


The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information.
-------------- next part --------------
An HTML attachment was scrubbed...
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------------------------------

Message: 4
Date: Fri, 7 Sep 2007 14:16:20 -0400
From: "Eric Moore"
Subject: RE: [Zbrafish] Request information for the Zebrafish breeding
tanks
To: "sridevi" ,

Message-ID:

Content-Type: text/plain; charset="us-ascii"

Hello Sridevi,



Marine Biotech offers several different types of breeding tanks. Please
email me directly and I'll send you some information. Thanks, Eric



Eric Moore

Territory Manager

Marine Biotech

54A West Dane St.

Beverly, MA 01915 USA

Toll Free US (888) 624-8265 x 132

International (978) 927-8720 x 132

Cell 978 578-2740

Fax 978 921-0231

[Only registered users see links. ]

________________________________

From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of sridevi
Sent: Friday, September 07, 2007 7:15 AM
To: [Only registered users see links. ]
Subject: [Zbrafish] Request information for the Zebrafish breeding tanks



Dear Sir/Madam,



I sridevi from India working as a Junior Research Fellow under Dr.
V.Kalarani Head, Associate Professor, Department of Biotechnology, Sri
Padmavati Women's University, Tirupati, Chittoor Dist., Andhra Pradesh,
India.

We are working on zebrafish. So we need the zebrafish breeding tanks.
Please give me the modalities and information for purchase of zebrafish
breeding tanks.



With Best Regards,



Sridevi



________________________________

size=1 width="100%" align=center>

5, 50, 500, 5000 - Store N number of mails in your inbox. Click here.
mail/yahoomail/tools/tools-08.html/>

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------------------------------

_______________________________________________
Zbrafish mailing list
[Only registered users see links. ]
[Only registered users see links. ]

End of Zbrafish Digest, Vol 28, Issue 3
***************************************



---------------------------------
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Hi Sridevi,
Depending upon whether you intend to do pair spawning or group spawning,you can use different types of breeding tanks. Plasic tanks of 2 lit capacity with a fitting trap to allow the eggs settle at the bottom will be sufficient for pair spawning where as 8 lit glass tank or fibre glass tank with suitable trap will serve the purpose for group spawning.
In both cases I would suggest you can make your traps or sieve we can say using plastic or fibre glass to fit the size of the tanks.The point here is, the eggs need to be clearly separated and settled down on the bottom of the tank to save it from being eaten up by the adults,meaning eggs can pass through the sieve while the adults should not.You can attach some plastic leaves or some soft substance to the sieve/trap as they love to lay the eggs around it!!!.............and your breeding tank is ready...

rds
Rajesh Krishnan
University of Toronto

[Only registered users see links. ] wrote:
Send Zbrafish mailing list submissions to
[Only registered users see links. ]

To subscribe or unsubscribe via the World Wide Web, visit
[Only registered users see links. ]
or, via email, send a message with subject or body 'help' to
[Only registered users see links. ]

You can reach the person managing the list at
[Only registered users see links. ]

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Zbrafish digest..."


Today's Topics:

1. Operon Zebrafish oligo probe set ([Only registered users see links. ])
2. Request information for the Zebrafish breeding tanks (sridevi)
3. RE: Request information for the Zebrafish breeding tanks
(christian lawrence)
4. RE: Request information for the Zebrafish breeding tanks
(Eric Moore)


----------------------------------------------------------------------

Message: 1
Date: Fri, 07 Sep 2007 07:17:26 -0700
From: [Only registered users see links. ]
Subject: [Zbrafish] Operon Zebrafish oligo probe set
To: [Only registered users see links. ]
Message-ID: <1189174646.952213.98410@d55g2000hsg.googlegroups. com>
Content-Type: text/plain; charset="iso-8859-1"

Hi everyone,

Operon is planning the release of a microarray ready probe set for
printing microarrays for Zebrafish. The set includes about 22,000 70
mer probes that cover the entire genome. If you or a colleague are
interested please feel free to contact me for more information.

Thank you,

Abria

[Only registered users see links. ]
256-704-8159



------------------------------

Message: 2
Date: Fri, 7 Sep 2007 12:15:05 +0100 (BST)
From: sridevi
Subject: [Zbrafish] Request information for the Zebrafish breeding
tanks
To: [Only registered users see links. ]
Message-ID: <[Only registered users see links. ].in.yahoo.com>
Content-Type: text/plain; charset="iso-8859-1"

Dear Sir/Madam,

I sridevi from India working as a Junior Research Fellow under Dr. V.Kalarani Head, Associate Professor, Department of Biotechnology, Sri Padmavati Women's University, Tirupati, Chittoor Dist., Andhra Pradesh, India.
We are working on zebrafish. So we need the zebrafish breeding tanks. Please give me the modalities and information for purchase of zebrafish breeding tanks.

With Best Regards,

Sridevi


---------------------------------
5, 50, 500, 5000 - Store N number of mails in your inbox. Click here.
-------------- next part --------------
An HTML attachment was scrubbed...
URL: [Only registered users see links. ]

------------------------------

Message: 3
Date: Fri, 7 Sep 2007 14:11:43 -0400
From: "christian lawrence"
Subject: RE: [Zbrafish] Request information for the Zebrafish breeding
tanks
To: "'sridevi'" ,

Message-ID:
Content-Type: text/plain; charset="us-ascii"

Most suppliers (AHAB, MBI, Tecniplast, etc.) make their own version of
breeding trap. Most of these, as well as others made by laboratory supply
companies, are plastic traps that fit inside plastic tanks.

In my opinion (based upon my personal experience, data collected in
laboratory settings, and observations of the animals spawning in the wild)
the ones that work best are those that can be "tipped", providing a shallow
to deep gradient within the trap. The fish appear to preferentially spawn
in the shallow areas. If you don't believe me, try it, and pay close
attention to how the fish behave and where most of the eggs are laid.

All cages will work. But those that can be easily tipped work better.
Still my opinion. But a semi-informed one.

With this in mind, we use 2 liter polycarbonate mouse cages from nalgene or
tecniplast, fill it with fish water, and insert the following breeding trap,
tip it, and let the fish do their thing (with proper conditioning, of
course).

[Only registered users see links. ]

Using such an approach, you can probably get all supplies in India (mouse
cages, reasonable facsimile of breeding trap).

Also you can check out
[Only registered users see links. ]
[Only registered users see links. ]
[Only registered users see links. ]
[Only registered users see links. ]

Good luck,
Chris



Christian Lawrence
Brigham and Women's Hospital
Karp Family Research Laboratories 06-004B
One Blackfan Circle
Boston, Massachusetts 02115
Tel: 617.355.9041
Fax: 617.355.9064




_____

From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of sridevi
Sent: Friday, September 07, 2007 7:15 AM
To: [Only registered users see links. ]
Subject: [Zbrafish] Request information for the Zebrafish breeding tanks


Dear Sir/Madam,

I sridevi from India working as a Junior Research Fellow under Dr.
V.Kalarani Head, Associate Professor, Department of Biotechnology, Sri
Padmavati Women's University, Tirupati, Chittoor Dist., Andhra Pradesh,
India.
We are working on zebrafish. So we need the zebrafish breeding tanks. Please
give me the modalities and information for purchase of zebrafish breeding
tanks.

With Best Regards,

Sridevi


_____

5, 50, 500, 5000 - Store N number of mails in your inbox. Click
l/yahoomail/tools/tools-08.html/> here.


The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information.
-------------- next part --------------
An HTML attachment was scrubbed...
URL: [Only registered users see links. ]

------------------------------

Message: 4
Date: Fri, 7 Sep 2007 14:16:20 -0400
From: "Eric Moore"
Subject: RE: [Zbrafish] Request information for the Zebrafish breeding
tanks
To: "sridevi" ,

Message-ID:

Content-Type: text/plain; charset="us-ascii"

Hello Sridevi,



Marine Biotech offers several different types of breeding tanks. Please
email me directly and I'll send you some information. Thanks, Eric



Eric Moore

Territory Manager

Marine Biotech

54A West Dane St.

Beverly, MA 01915 USA

Toll Free US (888) 624-8265 x 132

International (978) 927-8720 x 132

Cell 978 578-2740

Fax 978 921-0231

[Only registered users see links. ]

________________________________

From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of sridevi
Sent: Friday, September 07, 2007 7:15 AM
To: [Only registered users see links. ]
Subject: [Zbrafish] Request information for the Zebrafish breeding tanks



Dear Sir/Madam,



I sridevi from India working as a Junior Research Fellow under Dr.
V.Kalarani Head, Associate Professor, Department of Biotechnology, Sri
Padmavati Women's University, Tirupati, Chittoor Dist., Andhra Pradesh,
India.

We are working on zebrafish. So we need the zebrafish breeding tanks.
Please give me the modalities and information for purchase of zebrafish
breeding tanks.



With Best Regards,



Sridevi



________________________________

size=1 width="100%" align=center>

5, 50, 500, 5000 - Store N number of mails in your inbox. Click here.
mail/yahoomail/tools/tools-08.html/>

-------------- next part --------------
An HTML attachment was scrubbed...
URL: [Only registered users see links. ]

------------------------------

_______________________________________________
Zbrafish mailing list
[Only registered users see links. ]
[Only registered users see links. ]

End of Zbrafish Digest, Vol 28, Issue 3
***************************************



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