[Zbrafish] Request information for the Zebrafish breeding tanks

I think Chris is right, shallow water encourages spawning. We use the
Aquatic Habitats 2-liter breeder tanks but only fill them about a quarter
full (actually we fill them to maybe 80% when we set the fish up the night
before spawning, then the next morning transfer the insert + fish to a
quarter-filled tank of new water, remove the divider, wait.) Perhaps a
tipping/gradient strategy like Chris describes would be less stressful to
the fish though.

- Jim


_____

From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of christian
lawrence
Sent: Friday, September 07, 2007 2:12 PM
To: 'sridevi'; [Only registered users see links. ]
Subject: RE: [Zbrafish] Request information for the Zebrafish breeding tanks


Most suppliers (AHAB, MBI, Tecniplast, etc.) make their own version of
breeding trap. Most of these, as well as others made by laboratory supply
companies, are plastic traps that fit inside plastic tanks.

In my opinion (based upon my personal experience, data collected in
laboratory settings, and observations of the animals spawning in the wild)
the ones that work best are those that can be "tipped", providing a shallow
to deep gradient within the trap. The fish appear to preferentially spawn
in the shallow areas. If you don't believe me, try it, and pay close
attention to how the fish behave and where most of the eggs are laid.

All cages will work. But those that can be easily tipped work better.
Still my opinion. But a semi-informed one.

With this in mind, we use 2 liter polycarbonate mouse cages from nalgene or
tecniplast, fill it with fish water, and insert the following breeding trap,
tip it, and let the fish do their thing (with proper conditioning, of
course).

[Only registered users see links. ]

Using such an approach, you can probably get all supplies in India (mouse
cages, reasonable facsimile of breeding trap).

Also you can check out
[Only registered users see links. ]
[Only registered users see links. ]
[Only registered users see links. ]
[Only registered users see links. ]

Good luck,
Chris



Christian Lawrence
Brigham and Women's Hospital
Karp Family Research Laboratories 06-004B
One Blackfan Circle
Boston, Massachusetts 02115
Tel: 617.355.9041
Fax: 617.355.9064




_____

From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of sridevi
Sent: Friday, September 07, 2007 7:15 AM
To: [Only registered users see links. ]
Subject: [Zbrafish] Request information for the Zebrafish breeding tanks


Dear Sir/Madam,

I sridevi from India working as a Junior Research Fellow under Dr.
V.Kalarani Head, Associate Professor, Department of Biotechnology, Sri
Padmavati Women's University, Tirupati, Chittoor Dist., Andhra Pradesh,
India.
We are working on zebrafish. So we need the zebrafish breeding tanks. Please
give me the modalities and information for purchase of zebrafish breeding
tanks.

With Best Regards,

Sridevi



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[avdesh]

Hi Sridevi from India I recommend breeding tanks either from Aquatic habitats or you can order them in India as well and you can get them cusom made if you compare the price from USA. The concept is very simple you need a mess at the bottom of the tank which have to fit in other tank and you need to have a divider which can also be custom made from your local mechanic shop who deals with plastic.
Avdesh
Perth, Western Australia