positive control for toxicity study

Hi i'm a graduate student studying some wastewater using the zebrafish
embryo toxicity test and am unfamiliar with the development of the
embryos. I need a positive control substance to give known
malformations at known times so that I can compare it to normal
healthy development (just as background information before starting
the toxicity test). If anyone knows of a good substance, please
please let me know!

On May 17, 4:10 pm, [Only registered users see links. ] wrote:

Hi, in germany u are required to use 3,4-dichloroalinin as the
positive substance for waste water.
Stock solution: 0.05g of 3,4-dichloroalinin dissolved in 500ml fish
medium and 24h under agitation. pH must be 7.0. U can keep it up to 6
months in the fridge.
The final concentration is 3.7mg/L
hope it can help
regards,
francois