Morpholino injection concentrations and carriers?

Good day zebrafish investigators,

This is Jon from Gene Tools. I've a question about injection
concentrations used for Morpholino studies in zebrafish. Based on our
interactions with zebrafish researchers, we think that most folks use a
1 nanogram/nanoliter concentration of Morpholinos for their injections.
It seems water is the most common solvent, followed by Danieu buffer.
I would be very interested in learning more about the concentrations
and solutions now commonly in use. Are higher concentrations used, and
if so why and how often? What about stock solutions - is the usual
stock concentration about 1 milliMolar, and are stocks most commonly in
water? We are doing some systematic studies of Morpholino solubilities
and storage conditions and this information will help us to design
experiments.

To summarize the critical questions:
(1) what Morpholino concentration do you use for injections?
(2) what solution do you use in injections to carry the Morpholinos?
(3) what Morpholino concentration do you use for stocks?
(4) what solution do you use to dissolve Morpholinos in order to make
stocks?

Thanks in advance for any data you can help me with.

- Jon

Jon D. Moulton, Ph.D.
Diagnostics and Special Projects
GENE TOOLS, LLC
[Only registered users see links. ]
[Only registered users see links. ]
(541) 929-7840 x1201

(1) varies between 1mM (8.3ng/nl) to 1/6 mM
(2) Danieu buffer (water if co-inject with mRNA)
(3) 1mM
(4) Danieu buffer


-Haiqiong



On May 2, 5:04 pm, "Jon D.Moulton" <[Only registered users see links. ]> wrote:

On 3 mei, 23:48, Haiqiong <[Only registered users see links. ]> wrote:

Hi Haiqiong,

I was just wondering, is there a specific reason for you to use water
in stead of Danieu buffer for Morpholino / RNA co-injections? I have
been doing these in Danieu as well so far...

thanks in advance

-xaos