As far as I know, there is no true inbred zebrafish line at the
(if there is, we would also be quite interested to learn about it !).
Even the "really inbred" generated by gynogenesis or androgenesis had
be crossed to other lines to be maintained.
Currently a very good reference about this topic is the following
V. Guryev et al. "Genetic variation in the zebrafish", Genome
As we also use zebrafish as a model for immunology, we tried to
our own zebrafish inbred lines by standard brother-sister matings. As
we had to drop quite a few lines after some crosses because of squewed
ratios or loss of fertility.
However, we now have a few lines that have reached the 7th or 8th
They are not vigorous breeders and most of them have some problems
(females tending to be egg-bound very early or lots of abnormal
But they keep going, and at least one is fairly OK.
When they reach the tenth generation, we will make a careful
the remaining genetic diversity since genetic contamination is always
be suspected. Then, they should at last be usable!
Unité Macrophages et Développement de l'Immunité
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On Mar 22, 3:06 pm, "Mooij, M." <[Only registered users see links. ]> wrote: