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primary antibody

primary antibody - Zebrafish Forum

primary antibody - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 08-23-2006, 03:01 PM
Ralph
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Default primary antibody



I am not a member of the zebrafish research community. I do, however,
teach developmental biology and will use zebrafish in my > teaching
lab. I wish to do experiments on simple chemical
teratogenesis and would like to use whole mount indirect
immunofluorescence as the end point. My experience with this technique
is minimal and funds are limited, I seek a protocol and a
useful/inexpensive primary antibody to use. Any help?

Ralph Sorensen
Gettysburg College

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