Zebrafish RBC lysis

Hi Charlie,



You can try the following RBC lysis buffer : 144 mM NH4Cl, 17 mM Tris, pH7.2
(Betti et al., 1993).

This buffer is used in the comet assay protocol to eliminate ZF RBC from ZF
hepatocytes suspension.



Best wishes,

Valérie LARNO, PhD
Institut de Radioprotection et de Sûreté Nucléaire
DEI / SECRE / LRE - Bât 180
CE Cadarache BP 3
13115 Saint Paul Lez Durance
tél. 04 42 19 92 60
fax 04 42 19 91 51
[Only registered users see links. ] <mailto:[Only registered users see links. ]>




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Message: 1
Date: Mon, 26 Jun 2006 12:03:14 +0100
From: Charles Ellis
Subject: [Zbrafish] Zebrafish RBC lysis
To:
Message-ID:
Content-Type: text/plain; charset="US-ASCII"

Dear all,

Does anyone have a protocol to lyse zebrafish red blood cells? We have
tried
using mammalian RBC lysis kits but these were unsuccessful in lysing the
cells from the fish. Any help or suggestions would be appreciated.

Cheers,

Charlie Ellis.
Section of Immunology and Infection,
Division of Cell and Molecular Biology,
Sir Alexander Fleming Building,
Imperial College Road,
Imperial College, London.
SW7 2AZ UK.