Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Zebrafish Forum
Register Search Today's Posts Mark Forums Read

Zebrafish Forum Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


Zebrafish RBC lysis

Zebrafish RBC lysis - Zebrafish Forum

Zebrafish RBC lysis - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 06-29-2006, 07:30 AM
LARNO Valérie
Guest
 
Posts: n/a
Default Zebrafish RBC lysis



Hi Charlie,



You can try the following RBC lysis buffer : 144 mM NH4Cl, 17 mM Tris, pH7.2
(Betti et al., 1993).

This buffer is used in the comet assay protocol to eliminate ZF RBC from ZF
hepatocytes suspension.



Best wishes,

Valérie LARNO, PhD
Institut de Radioprotection et de Sûreté Nucléaire
DEI / SECRE / LRE - Bât 180
CE Cadarache BP 3
13115 Saint Paul Lez Durance
tél. 04 42 19 92 60
fax 04 42 19 91 51
[Only registered users see links. ] <mailto:[Only registered users see links. ]>




----------------------------------------------------------------------

Message: 1
Date: Mon, 26 Jun 2006 12:03:14 +0100
From: Charles Ellis
Subject: [Zbrafish] Zebrafish RBC lysis
To:
Message-ID:
Content-Type: text/plain; charset="US-ASCII"

Dear all,

Does anyone have a protocol to lyse zebrafish red blood cells? We have
tried
using mammalian RBC lysis kits but these were unsuccessful in lysing the
cells from the fish. Any help or suggestions would be appreciated.

Cheers,

Charlie Ellis.
Section of Immunology and Infection,
Division of Cell and Molecular Biology,
Sir Alexander Fleming Building,
Imperial College Road,
Imperial College, London.
SW7 2AZ UK.




Reply With Quote
Reply

Tags
lysis , rbc , zebrafish


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Zebrafish PKA Tyrone Zebrafish Forum 1 09-19-2013 01:24 PM
Zbrafish Digest, Vol 28, Issue 3 raj abi Zebrafish Forum 0 09-07-2007 07:35 PM
Zebrafish RBC lysis Charles Ellis Zebrafish Forum 0 06-26-2006 11:03 AM
Ocimu Biosolutions is offering Zebrafish microarrays (Zebrafish 14K OciChip™) biosoftrex Zebrafish Forum 0 01-30-2006 12:57 PM
red pigment in zebrafish christian lawrence Zebrafish Forum 0 03-31-2005 06:12 PM


All times are GMT. The time now is 10:07 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11529 seconds with 16 queries