I have been trying to get hold of the pcGlobin 2 and pcGlobin 2-GST
vectors for stable mRNA synthesis for zebrafish (see abstract below) but
without success. Can anyone send me a working email address for these
researchers or possibly supply me with the plasmids themselves?
Regards and thanks,
Mol Cells. 2004 Apr 30;17(2):373-6.
Novel vector systems optimized for injecting in vitro-synthesized mRNA into
Ro H, Soun K, Kim EJ, Rhee M.
School of Biosciences and Biotechnology, Chungnam National University,
Microinjection of nucleic acids or proteins is a useful way of studying
embryonic development. In particular, injection of in vitro-transcribed
RNA is commonly employed to achieve ectopic or increased expression of genes.
Two vector systems, pCS2+ and pT7Ts, have been used for this purpose in
zebrafish. However, they were initially optimized for Xenopus embryos not
zebrafish. Here we describe a vector, pcGlobin2, optimized for
its derivative, pcGlobin2-GST. This new vector system offers several
First, pcGlobin 2 contains three critical elements 15' and 3' zebrafish
beta-globin UTRs, and a poly(A) tail] for generating stable mRNAs and
improving translation efficiency. Second, subcloning and preparation of
DNA is easier because of the larger number of restriction sites. Third,
protein-binding assays can be performed directly on the injected embryos
pcGlobin2-GST. Lastly, this vector system can be transfected into animal
without additional subcloning.
PMID: 15179057 [PubMed - indexed for MEDLINE]
Discipline of Genetics and
Special Research Centre for the
Molecular Genetics of Development
School of Molecular and Biomedical Science
The University of Adelaide
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