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#1
05-17-2005, 07:14 PM
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 ELF 97 In Situ Hybridization Kit Protocol -------------------------------------------------------------------------- Has anyone used the ELF 97 In Situ Hybridization Kit to detect hybridized riboprobes in zebrafish embryos? I am looking for a protocol to follow. Specifically, I am trying to label one probe with fast red, and then label a second probe with the ELF kit so that I can do 2 color fluorescent imaging. Also, is 70% glycerol suitable for mounting embryos that have been subjected to the ELF reagents or will the precipitate "leak out"? Thanks! Marc Wolman Halloran Laboratory University of Wisconsin-Madison ---  |
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| elf , hybridization , kit , protocol , situ |
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