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ELF 97 In Situ Hybridization Kit Protocol

ELF 97 In Situ Hybridization Kit Protocol - Zebrafish Forum

ELF 97 In Situ Hybridization Kit Protocol - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 05-17-2005, 07:14 PM
Marc Wolman
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Default ELF 97 In Situ Hybridization Kit Protocol



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Has anyone used the ELF 97 In Situ Hybridization Kit to detect
hybridized riboprobes in zebrafish embryos? I am looking for a
protocol to follow. Specifically, I am trying to label one probe with
fast red, and then label a second probe with the ELF kit so that I can do
2 color fluorescent imaging. Also, is 70% glycerol suitable for mounting
embryos that have been subjected to the ELF reagents or will the
precipitate "leak out"? Thanks!

Marc Wolman
Halloran Laboratory
University of Wisconsin-Madison

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