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sectioning - Zebrafish Forum

sectioning - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.

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Old 04-04-2005, 04:45 PM
Jeffrey Hannah
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Default sectioning


Thanks for responding everyone, I certainly appreciate it. Here are the
responses I have received so far:

Hi Jeffrey. For frogs and fish, the yolk tends to crumble the wax.
more tedious, but gives beautiful results.-

I wonder if the variability has anything to do with how much yolk is in
the particular section? Yolk-free sections, say from the head or caudal
tail of a 24h fish will be fine w/ paraffin, but as soon as you get to
the middle where the yolk or yolk extension is included, you run into
problems with holes in the wax.

-Ben Feldman ([Only registered users see links. ].gov)

Hi Jeffrey,
We have been using JB4 resin to section in situ embryos and we are getting
really nice results. We can even take pictures and do H&E afterwards to see
histology of the section.

-Rebecca Burdine ([Only registered users see links. ])


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