Thanks for responding everyone, I certainly appreciate it. Here are the
responses I have received so far:
Hi Jeffrey. For frogs and fish, the yolk tends to crumble the wax.
more tedious, but gives beautiful results.-
I wonder if the variability has anything to do with how much yolk is in
the particular section? Yolk-free sections, say from the head or caudal
tail of a 24h fish will be fine w/ paraffin, but as soon as you get to
the middle where the yolk or yolk extension is included, you run into
problems with holes in the wax.
-Ben Feldman ([Only registered users see links. ].gov)
We have been using JB4 resin to section in situ embryos and we are getting
really nice results. We can even take pictures and do H&E afterwards to see
histology of the section.
-Rebecca Burdine ([Only registered users see links. ])