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Zebrafish Forum Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


sectioning

sectioning - Zebrafish Forum

sectioning - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 04-01-2005, 09:14 PM
Jeffrey Hannah
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Default sectioning



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Hello everyone,
After whole-mount in situs, I often section my zebrafish after
embedding them in paraffin. Despite the fact that I follow the same
protocol and use the same sectioning methods for all the embryos, many
times there are problems with some of them. My most common problem is
that the embryos will often crumble and crack. I have tried isolating the
problem, but I can't seem to figure it out. Does anyone use
paraffin for zebrafish sections? If so, have you run into this problem
and could I see your protocol? Do plastic or cryogenic sectioning work
better for whole-mount in situ zebrafish? Or am I better off performing
in situ hybridization after sectioning? Any advice is appreciated.

Jeffrey Hannah
Children's Hospital of Philadelphia

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