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transgenic muscle rfp/bfp/yfp expressers

transgenic muscle rfp/bfp/yfp expressers - Zebrafish Forum

transgenic muscle rfp/bfp/yfp expressers - Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.


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Old 04-03-2004, 01:17 AM
Thomas E. Hall
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Default transgenic muscle rfp/bfp/yfp expressers



Hi,

I am trying to find a zebrafish trangenic line that expresses rfp, bfp
or yfp ideally under the control of the skeletal muscle a-actin
promoter (I currently have the gfp one but need a second colour for some
complex transplant experiments). Does anyone have, or know of such a line?

As a second option it would also be possible to use the yfp/rfp fish
recently developed with the myosin light chain promoter. Can anyone
recommend a good supplier, bearing in mind we would have to ship to
Australia?

Thanks,

Tom Hall


Dr. Thomas E. Hall
Muscle Development Laboratory (Currie Lab)
Victor Chang Cardiac Research Institute
Sydney
NSW 2010
AUSTRALIA
---


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Old 07-21-2011, 03:10 PM
Pipette Filler
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Smile Re: transgenic muscle rfp/bfp/yfp expressers

Dear Dr. Tom Hall,

How is your experiment going on?

I am in Pukyong National University, Korea. We have been doing marine medaka Oryzias dancena experiments with GFP, RFP, CFP and BFP reporter driven by MLC - 2 (Myosin light chain 2) promoter. And we are successfully generation stable line this fish up to F4. If you are interested in this topic, please take a free to contact with me.

NGUYEN THANH VU (워 탄 부)

Institute of Marine Living Modified Organisms (IMLMO)
Risk Assessment Center for Marine LMO by MLTMA
Pukyong National University (PKNU), Nam-gu 599-1, Busan 608-737
Korea, Republic of.


Quote:
Originally Posted by Thomas E. Hall View Post
Hi,

I am trying to find a zebrafish trangenic line that expresses rfp, bfp
or yfp ideally under the control of the skeletal muscle a-actin
promoter (I currently have the gfp one but need a second colour for some
complex transplant experiments). Does anyone have, or know of such a line?

As a second option it would also be possible to use the yfp/rfp fish
recently developed with the myosin light chain promoter. Can anyone
recommend a good supplier, bearing in mind we would have to ship to
Australia?

Thanks,

Tom Hall


Dr. Thomas E. Hall
Muscle Development Laboratory (Currie Lab)
Victor Chang Cardiac Research Institute
Sydney
NSW 2010
AUSTRALIA
---
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expressers , muscle , rfp or bfp or yfp , transgenic


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