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ade2-101 ochre mutation

ade2-101 ochre mutation - Yeast Forum

ade2-101 ochre mutation - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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Old 02-07-2013, 12:35 PM
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Default ade2-101 ochre mutation



Dear all,

Does anyone could help me to understand why a S.cerevisiae strain carrying the ade2-101 ochre mutation becomes pink in YPD but not in YPEG?

Thanks a lot for your help!

Catarina
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Old 02-15-2013, 09:55 PM
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Default Re: ade2-101 ochre mutation

Quote:
Originally Posted by Cataripi View Post
Dear all,

Does anyone could help me to understand why a S.cerevisiae strain carrying the ade2-101 ochre mutation becomes pink in YPD but not in YPEG?

Thanks a lot for your help!

Catarina
Have you tried simply growing it longer, perhaps it grows slower in the YPEG?

I have noticed when plating these things that on YPD sometimes it takes a bit of time for the red color to develop on the colonies.

Right now we are growing basically the exact same thing, W303 in YPEG, and they definitely are red.
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