ade2-101 ochre mutation

[Cataripi]

Dear all,

Does anyone could help me to understand why a S.cerevisiae strain carrying the ade2-101 ochre mutation becomes pink in YPD but not in YPEG?

Thanks a lot for your help!

Catarina

[pigfarmer]

Originally Posted by Cataripi

Dear all,

Does anyone could help me to understand why a S.cerevisiae strain carrying the ade2-101 ochre mutation becomes pink in YPD but not in YPEG?

Thanks a lot for your help!

Catarina

Have you tried simply growing it longer, perhaps it grows slower in the YPEG?

I have noticed when plating these things that on YPD sometimes it takes a bit of time for the red color to develop on the colonies.

Right now we are growing basically the exact same thing, W303 in YPEG, and they definitely are red.