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Cataripi 02-07-2013 12:35 PM

ade2-101 ochre mutation
 
Dear all,

Does anyone could help me to understand why a S.cerevisiae strain carrying the ade2-101 ochre mutation becomes pink in YPD but not in YPEG?

Thanks a lot for your help!

Catarina

pigfarmer 02-15-2013 09:55 PM

Re: ade2-101 ochre mutation
 
Quote:

Originally Posted by Cataripi (Post 445178)
Dear all,

Does anyone could help me to understand why a S.cerevisiae strain carrying the ade2-101 ochre mutation becomes pink in YPD but not in YPEG?

Thanks a lot for your help!

Catarina

Have you tried simply growing it longer, perhaps it grows slower in the YPEG?

I have noticed when plating these things that on YPD sometimes it takes a bit of time for the red color to develop on the colonies.

Right now we are growing basically the exact same thing, W303 in YPEG, and they definitely are red.


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