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ade2-101 ochre mutation

Dear all,

Does anyone could help me to understand why a S.cerevisiae strain carrying the ade2-101 ochre mutation becomes pink in YPD but not in YPEG?

Thanks a lot for your help!

Catarina

Re: ade2-101 ochre mutation

Quote:

Originally Posted by Cataripi (Post 445178)

Dear all,

Does anyone could help me to understand why a S.cerevisiae strain carrying the ade2-101 ochre mutation becomes pink in YPD but not in YPEG?

Thanks a lot for your help!

Catarina

Have you tried simply growing it longer, perhaps it grows slower in the YPEG?

I have noticed when plating these things that on YPD sometimes it takes a bit of time for the red color to develop on the colonies.

Right now we are growing basically the exact same thing, W303 in YPEG, and they definitely are red.