Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Yeast Forum
Register Search Today's Posts Mark Forums Read

Yeast Forum Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


Small yeast colonies on 2-hybrid screening plates

Small yeast colonies on 2-hybrid screening plates - Yeast Forum

Small yeast colonies on 2-hybrid screening plates - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-13-2012, 12:26 PM
Pipette Filler
Points: 14, Level: 1 Points: 14, Level: 1 Points: 14, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2012
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Exclamation Small yeast colonies on 2-hybrid screening plates



Dear All,

I am doing some Y2H screens (with intrabody libraries) with the L40 strain (his3 -). I have a problem: when I plate onto selective -WHL + 3AT media, colonies do not grow well and remain small. What could it be? I tried with a positive control (a previously isolated intrabody binding the LexA DBD and the growth is very good onto same plates).
I am using a controlled bait line.

Your help is appreciated, thank you very much

Michele
Reply With Quote
  #2  
Old 09-20-2012, 11:12 PM
pigfarmer's Avatar
Summer Student
Points: 3,196, Level: 36 Points: 3,196, Level: 36 Points: 3,196, Level: 36
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2009
Location: Indiana
Posts: 99
Thanks: 26
Thanked 14 Times in 14 Posts
Default Re: Small yeast colonies on 2-hybrid screening plates

Quote:
Originally Posted by Michele88 View Post
Dear All,

I am doing some Y2H screens (with intrabody libraries) with the L40 strain (his3 -). I have a problem: when I plate onto selective -WHL + 3AT media, colonies do not grow well and remain small. What could it be? I tried with a positive control (a previously isolated intrabody binding the LexA DBD and the growth is very good onto same plates).
I am using a controlled bait line.

Your help is appreciated, thank you very much

Michele
How do youmean the colonies are small? Do the cells grow well prior to mating? Maybe there just isn't any interaction, or very slight?
Reply With Quote
Reply

Tags
2hybrid , colonies , plates , screening , small , yeast


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
yeast one hybrid - beta-gal assay inge Yeast Forum 1 06-18-2009 07:20 PM
New Saccharomyces Sequences 09/08/04 SGD Sequences Yeast Forum 0 09-13-2004 10:07 PM
New Saccharomyces Sequences 08/11/04 SGD Sequences Yeast Forum 0 08-12-2004 12:26 AM
yeast two hybrid Alexandra Blak Yeast Forum 0 08-27-2003 06:02 PM
yeast two hybrid simona.baldassa@unimi.it Yeast Forum 0 08-26-2003 11:47 PM


All times are GMT. The time now is 09:01 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13428 seconds with 16 queries