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Small yeast colonies on 2-hybrid screening plates

Dear All,

I am doing some Y2H screens (with intrabody libraries) with the L40 strain (his3 -). I have a problem: when I plate onto selective -WHL + 3AT media, colonies do not grow well and remain small. What could it be? I tried with a positive control (a previously isolated intrabody binding the LexA DBD and the growth is very good onto same plates).
I am using a controlled bait line.

Your help is appreciated, thank you very much

Michele

Re: Small yeast colonies on 2-hybrid screening plates

Quote:

Originally Posted by Michele88 (Post 442078)

How do youmean the colonies are small? Do the cells grow well prior to mating? Maybe there just isn't any interaction, or very slight?