Hi, I wanted to find out if anyone has tried using fructose instead of raffinose, sucrose, lactic acid or glycerol in synthetic dropout media (or rich YEP medium) to grow yeast cells prior to galactose induction. I am trying to do galactose shifts on synchronized populations but releasing cells from raffinose or sucrose dropout medium into galactose dropout medium causes the cells to freeze and not resume budding until many hours later (~6).
On the other hand, the GAL1 promoter is noticeably leaky in lactic acid medium and the cells grow very slowly.
I have my reasons to suspect fructose will work but I wanted to ask if anyone has had prior experience.
Thank you all.