Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Yeast Forum
Register Search Today's Posts Mark Forums Read

Yeast Forum Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


Fructose as a non-inducing carbon source prior to galactose induction

Fructose as a non-inducing carbon source prior to galactose induction - Yeast Forum

Fructose as a non-inducing carbon source prior to galactose induction - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 07-23-2012, 07:36 PM
Pipette Filler
Points: 1,777, Level: 25 Points: 1,777, Level: 25 Points: 1,777, Level: 25
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2010
Posts: 15
Thanks: 0
Thanked 5 Times in 5 Posts
Default Fructose as a non-inducing carbon source prior to galactose induction



Hi, I wanted to find out if anyone has tried using fructose instead of raffinose, sucrose, lactic acid or glycerol in synthetic dropout media (or rich YEP medium) to grow yeast cells prior to galactose induction. I am trying to do galactose shifts on synchronized populations but releasing cells from raffinose or sucrose dropout medium into galactose dropout medium causes the cells to freeze and not resume budding until many hours later (~6).

On the other hand, the GAL1 promoter is noticeably leaky in lactic acid medium and the cells grow very slowly.

I have my reasons to suspect fructose will work but I wanted to ask if anyone has had prior experience.

Thank you all.
Reply With Quote
  #2  
Old 08-28-2012, 09:40 PM
Pipette Filler
Points: 32, Level: 1 Points: 32, Level: 1 Points: 32, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2012
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Fructose as a non-inducing carbon source prior to galactose induction

I'm curious what you find out with using fructose...any luck with gene induction?
Reply With Quote
  #3  
Old 09-05-2012, 03:50 PM
Pipette Filler
Points: 1,777, Level: 25 Points: 1,777, Level: 25 Points: 1,777, Level: 25
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2010
Posts: 15
Thanks: 0
Thanked 5 Times in 5 Posts
Default Re: Fructose as a non-inducing carbon source prior to galactose induction

I haven't checked by western for actualy protein expression but judging by change in cell morphology, it seems the fructose to galactose shift for my wild-type strain is worse than the shift from the other non-inducing carbon sources I've used.

Leaky expression at high cell density is noticeably reduced in fructose medium compared with sucrose or raffinose media. It might be worth doing a western. Does anyone else have experience with fructose to galactose shifts?
Reply With Quote
The Following User Says Thank You to mkc3 For This Useful Post:
pigfarmer (09-06-2012)
Reply

Tags
carbon , fructose , galactose , induction , noninducing , prior , source


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Slow galactose induction (Kerry Geiler) Wang, Xin-Xiang Yeast Forum 0 09-05-2007 06:29 PM
[Yeast] Slow galactose induction Z.J. Zhang Yeast Forum 0 09-04-2007 09:27 PM
the absolutely final complete collection of ideas blochee Physics Forum 2 06-15-2007 06:31 AM
the absolutely final complete collection of ideas blochee Physics Forum 0 06-14-2007 10:30 PM
Yeast stop to grow after galactose induction Nicolas Brandes Yeast Forum 0 03-04-2006 10:18 PM


All times are GMT. The time now is 05:24 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.15416 seconds with 16 queries