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Yeast Forum Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


mRNA extraction

mRNA extraction - Yeast Forum

mRNA extraction - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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Old 11-15-2011, 08:20 PM
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Default mRNA extraction



Hi all,

I am wondering if anyone out there does any qrt-pcr and has a good method for preparation of total RNA from yeast. I have a pretty good idea what I want to do, and I think I will try it tomorrow, but my Phenol:Chloroform is pH 7-8. I guess I can just add acid to it to lower the pH?
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