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Yeast cultures: Plates vs. Suspensions

Yeast cultures: Plates vs. Suspensions - Yeast Forum

Yeast cultures: Plates vs. Suspensions - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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Old 02-09-2011, 01:36 PM
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Default Yeast cultures: Plates vs. Suspensions



Hello everybody!

Situation:
I woild like to perform time-lapse microscopy with our yeast models to determine the most suitable time points for protein extraction using particular GFP-tagged proteins. I would like to use these data to collect more sample material from large yeast suspensions for further analysis.

Question:
Is there a difference between yeast growth and behavior when comparing solid cultures to suspensions, in other words can I translate the points of time from my microscopy results to bigger amount of cells in a suspension?

It would be nice if you could give me some advice, share your experience or recommend some literature.
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Old 02-10-2011, 08:01 AM
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Default Re: Yeast cultures: Plates vs. Suspensions

Hola, I think that there are differences, in suspension the disponibility of nutrients and O2 is easier. As you will culture the yeast in suspension, the microscope approach on liquid media will be more similar. Buena suerte
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Old 02-10-2011, 10:22 AM
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Default Re: Yeast cultures: Plates vs. Suspensions

Thank you very much,
do you or somebody else have suggestions for literature or suggestions where to look up experiences of other people?
THX

Last edited by Andrej; 02-11-2011 at 12:13 PM.
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Old 06-18-2012, 06:30 AM
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Default Re: Yeast cultures: Plates vs. Suspensions

learning
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cultures , plates , suspensions , yeast


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