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Yeast Forum Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


Double counter selection media

Double counter selection media - Yeast Forum

Double counter selection media - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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Old 08-30-2010, 05:51 PM
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Default Double counter selection media



I'm trying to make alpha-aminoadipate/5-fluoroanthranilic acid plates for counter selection of Lys+/Trp+ yeast, but I can't find a recipe anywhere. Looking at the individual alpha-aa and 5-FAA recipes is a bit confusing; I'm not sure if I need to supplement with additional amino acids, ammonium sulfate, etc.

Does anyone have a recipe?
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Old 09-07-2010, 02:29 PM
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Default Re: Double counter selection media

I have never worked with FAA, but I would assume you want to use MSG instead of Ammonium Sulfate...
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