Very new in yeast field & needs an urgent help.
I need 2 introduce His3 resistance into yeast strain & want 2 know if it is possible to make transformation of PCR product to get resistance with no plasmid. If so what should the PCR primers include besides homology to the His3 gene in order to dig it out of genomich yeast DNA?
2. Can the PCR piece be than directly transformed into the wanted strain without the use of a plasmid?
3. If so what is the principle this transformation is working upon?
Thanks a lot