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yeast plates with oleic acid

yeast plates with oleic acid - Yeast Forum

yeast plates with oleic acid - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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  #1  
Old 06-23-2009, 07:45 PM
Stephanie Kaut
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Default yeast plates with oleic acid



Hello together,

I want to test some yeast strains for growth on YPAD plates with oleic
acid.
But preparation of the plates seems to be tricky, because oleic acid and
the agar don't mix well, and the oleic acid swims on the top of the agar.
What can I do to have nice plates?

Best regards,
Stephanie

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Old 11-03-2009, 01:40 PM
Pipette Filler
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Default Re: yeast plates with oleic acid

Hi!

I use 0.1 % oleic acid that I mix with a pre-warmed (at 50) 1 % Triton X100 solution. You should mix it by inverting, very slowly. Once the oleate is mixed, I add it to my agar solution.
Here is a reference : Lockshon D, et al. (2007) The sensitivity of yeast mutants to oleic Acid implicates the peroxisome and other processes in membrane function. Genetics 175(1):77-91

Good luck!
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