yeast plates with oleic acid

Hello together,

I want to test some yeast strains for growth on YPAD plates with oleic
acid.
But preparation of the plates seems to be tricky, because oleic acid and
the agar don't mix well, and the oleic acid swims on the top of the agar.
What can I do to have nice plates?

Best regards,
Stephanie

[mnmaria]

Hi!

I use 0.1 % oleic acid that I mix with a pre-warmed (at 50°) 1 % Triton X100 solution. You should mix it by inverting, very slowly. Once the oleate is mixed, I add it to my agar solution.
Here is a reference : Lockshon D, et al. (2007) The sensitivity of yeast mutants to oleic Acid implicates the peroxisome and other processes in membrane function. Genetics 175(1):77-91

Good luck!