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Yeast Forum Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


Liquid media?

Liquid media? - Yeast Forum

Liquid media? - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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  #1  
Old 05-22-2009, 10:08 PM
Thomas Davis
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Default Liquid media?



Hi again everyone, and thanks for all the previous responses to my growth
rate question.

We have already tried to assess yeast growth rates using OD600 in TSB
(tryptic soy broth) media. We got rather spurious results, with the OD
jumping all over the place between measurements. What is the appropriate
liquid media to use for Pichia, Candida, and Saccharomyces?

We are worried that our previous dubious results may be due to using the
wrong kind of liquid media.

Once again, any advice on the appropriate media/technique would be greatly
appreciated.

Thanks again,

Seth Davis

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  #2  
Old 05-23-2009, 05:02 PM
Pipette Filler
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Default Re: Liquid media?

Saccharomyces and Candida can be well grown in YEPD or any rich media.You can get the recepie just by google YEPD OR YEPG.Generally sacchromyces grows well in those media.
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Subit
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