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-   -   Liquid media? (http://www.molecularstation.com/forum/yeast-forum/66303-liquid-media.html)

Thomas Davis 05-22-2009 10:08 PM

Liquid media?
 
Hi again everyone, and thanks for all the previous responses to my growth
rate question.

We have already tried to assess yeast growth rates using OD600 in TSB
(tryptic soy broth) media. We got rather spurious results, with the OD
jumping all over the place between measurements. What is the appropriate
liquid media to use for Pichia, Candida, and Saccharomyces?

We are worried that our previous dubious results may be due to using the
wrong kind of liquid media.

Once again, any advice on the appropriate media/technique would be greatly
appreciated.

Thanks again,

Seth Davis


subit 05-23-2009 05:02 PM

Re: Liquid media?
 
Saccharomyces and Candida can be well grown in YEPD or any rich media.You can get the recepie just by google YEPD OR YEPG.Generally sacchromyces grows well in those media.
Thanks
Subit


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