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Yeast Forum Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


determining growth rate

determining growth rate - Yeast Forum

determining growth rate - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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  #1  
Old 05-21-2009, 01:15 AM
Thomas Davis
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Default determining growth rate



Hi everyone,

I am an entomologist trying to study the microbial community associated
with an herbivorous beetle species. As such, our lab is rather limited in
terms of microbiological sophistication. We have tentatively isolated a
wealth of yeast colonies from the glandular mycangia of these insects, and
would like to conduct some performance-type experiments using our yeast
isolates and chemical treatments. Our isolations are currently being done on
2% MEA, and appear to mostly be in the genera Pichia, Saccharomyces, and
Candida.

My question to the group is this:

What is a reliable, quick, and easily interpreted method for assessing
growth rates of yeast colonies? We are familiar with the technique of colony
forming unit count via serial dilutions, however, this seems to be
a laborious means of assessing growth rate over time with many strains and
(potentially) multiple chemical treatments. Or is it simply best to try to
obtain a single 'snapshot' of growth rate?

Any advice that could be offered would be greatly appreciated. Thank you.

Seth Davis

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Old 05-23-2009, 04:57 PM
Pipette Filler
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Default Re: determining growth rate

I think you can grow single colony from saccharomyces in regular rich media(example-YEPD) and intially just track the growth rate simply by measuring O.D(example O.D:600) in spec.It will provide some idea regarding the growth in liquid media at various time point. At the same time you can go for counting the number of cells(by diluting) under microscope which will give idea of number of cells in individual O.D.
Thanks
Subit
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