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House keeping gene for Realtime PCR

House keeping gene for Realtime PCR - Yeast Forum

House keeping gene for Realtime PCR - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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Old 04-29-2009, 07:09 AM
Pipette Filler
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Default House keeping gene for Realtime PCR



I am trying to see expression of a common gene in 4 different saccharomyces cerevisiae strains.But the problem is my housekeeping gene(two) which I selected is giving variation of Ct value in each strains. Is ther any one who gone through same experience?
Any idea of a stable house keeping gene, irrespective of strains or phase(log vs stationary).
Thanks
Subit
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