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Cheap yeast extract/ proteose peptone for YPD?

Cheap yeast extract/ proteose peptone for YPD? - Yeast Forum

Cheap yeast extract/ proteose peptone for YPD? - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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  #1  
Old 12-03-2008, 03:42 AM
Andrew Carter
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Default Cheap yeast extract/ proteose peptone for YPD?



Dear All
I saw a thread similar to my current question back in the
mid 90s, but was wondering if anyone has an update.
We currently buy yeast extract and proteose peptone from
Becton Dickinson (via Fisher) for making YPD. However I
am beginning to do some large scale fermenter growths and
the cost is getting prohibitive. I notice that there are
much cheaper versions available (eg Sigma yeast extract
REF 09182 is almost 5 times cheaper per kg than Bacto
Yeast Extract). My question is whether anyone can
recommend a good (and cheap) combination of yeast extract
and proteose peptone that allows normal yeast growth (to a
similar OD etc).

Alternatively does anyone have any good ideas about cheap
media receipes for growing yeast to very high density in a
fed-batch fermenter run? Anyone ever tried using malt
extract (from a brew shop - seems cheap) for a protein
expression related project?

Many thanks and best wishes
Andrew

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  #2  
Old 12-19-2008, 05:03 PM
Pipette Filler
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Default Re: Cheap yeast extract/ proteose peptone for YPD?

Dear Andrew,
We can supply you very cost effective Peptones as nutrients.

We are glad to introduce ourselves as one of the leading Manufacturer & Exporter of Hydrolyzed Proteins based products
& Bio Nutrients - Peptones such as

1.Protein hydrolyzates in Liquid, Powder form derived from Casein,Soy Origin
2.Peptones for fermentation industry & Bacteriological Media Ingredients,
3.Amino Acid Chelates (Organic Trace Minerals) for human,animal nutrition.

Our products are presently exported in Europe, Middle East, African Countries for use in human, animal nutrition & as media ingredient.

We produce Hydrolyzed proteins, amino acids using ENZYMATIC HYDROLYSIS from of Proteins. We have a modern plant & huge capacity to produce Liquid & Spray Dried Powder of Peptones, Protein Hydrolyzates
& mineral amino acid chelates in India.

We are giving below the list of following products which are used as Bio Nutrients.
PEPTONES FOR CELL NUTRITION /FERMATATION /MEDIA INGREDIENTS -

Animal Free Peptones/Protein Hydrolyzates
Vegi PepS : Soy Peptone –NON ANIMAL,NON GMO
Vegi PepG : Peanut Peptone –NON ANIMAL,NON GMO

Animal Origin Peptones/Protein Hydrolyzates
NutriPep Bacto Peptone
Nutri Pep Casein Peptone
Nutri Pep Meat Peptone
Nutri Pep Proteose Peptone
Nutri Pep Pancreatic Digest Of Casein
NutriPep Acid Hydrolyzate of Casein Tech/Pure
Nutri Pep Tryptose
Nutri Pep Tryptone.
Nutri Pep Meat Extract Powder
Nutri Pep Yeast Extract Powder
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  #3  
Old 02-07-2009, 11:40 AM
Ph.D Doctorate
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Default Re: Cheap yeast extract/ proteose peptone for YPD?

This is something you can and should determine on your own, matt. Run the OD's, growth curves, assays for the objective - take responsibility for your science.
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Old 05-19-2009, 08:22 AM
Pipette Filler
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Default Re: Cheap yeast extract/ proteose peptone for YPD?

Dear Sir,

We export Yeast Extract and Peptone since 2001.
As an extract of autolyzed yeast cells, Yeast extract is a mixture of amino acids, peptides, water soluble vitamins and carbohydrates and can be used as additive for culture media. Also used in ham, sausage production and spread on toast or sandwiches at home foods as a natural favoring.

This product is full of VB1,VB2,VB6,VB12,niconacid folvite, pantothen biotin, and contains 19
kinds of amino acid and 5’ nucleotide etc.

Specification:
Apperance: light yellowish--brown yellowish fine powder
Total nitrogen: 9.0% min
а-amino nitrogen: 2.6% min
Loss on drying: 8.0% max
Ph: (5% solution) 5.0-6.0
Chloride: 5.0% max
Insoluble matter: 8.0% max
Ash: 15.0% max
Packing:25kg cardboard drum with inner double pe bag.
 

Our Peptones are made from bovine bone with meat. They have high protein, high activity, medium phosphorus, few moisture and ash etc characters . Edible Peptone conforms to sanitary standards in microbiology aspects.
They are widely used as microbiology culture media, nutriment additives, edible fungus
culture media and in medicine industry.

Peptone Reagent grade
Appearance: Yellow –brown powder
Specification: Total Nitrogen: 14.5 % min
Amino nitrogen: 2.5% ---3.0%
Water : 7% max
Ash: 6% max
PH : 5-7
Packing: 25kg cardboard drum


Best regards
Mike
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