[Yeast] 2 micron plasmid extraction

Hi all,

I'm looking to extract naturally occurring 2 micron plasmid from yeast.
There seem to be a range of plasmid DNA preps but having tried the qiagen
plasmid kit and failed i would appreciate a recommendation before buying
into different kits.

Does anyone have any experience with this?

All help much appreciated

Ellie

PS. thanks for all replies regarding aAA selection- please feel free to
contact me if interested in the results!
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[anubis2005]

The Zymoprep™ Yeast Plasmid Miniprep II I heard is a very good kit.

[pigfarmer]

If you still do, or anyone else want an easy prep for Plasmid from yeast let me know and I will post it, no kit needed, just too lazy to look for something that you probably already did 2.5 months ago!

[hairongxu88]

learning