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Yeast Forum Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


[Yeast] 2 micron plasmid extraction

[Yeast] 2 micron plasmid extraction - Yeast Forum

[Yeast] 2 micron plasmid extraction - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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  #1  
Old 08-15-2008, 11:25 AM
Ellie Harrison
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Default [Yeast] 2 micron plasmid extraction




Hi all,

I'm looking to extract naturally occurring 2 micron plasmid from yeast.
There seem to be a range of plasmid DNA preps but having tried the qiagen
plasmid kit and failed i would appreciate a recommendation before buying
into different kits.

Does anyone have any experience with this?

All help much appreciated

Ellie

PS. thanks for all replies regarding aAA selection- please feel free to
contact me if interested in the results!
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  #2  
Old 05-28-2011, 06:40 AM
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Default Re: [Yeast] 2 micron plasmid extraction

The Zymoprep™ Yeast Plasmid Miniprep II I heard is a very good kit.
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Old 11-15-2011, 08:23 PM
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Default Re: [Yeast] 2 micron plasmid extraction

If you still do, or anyone else want an easy prep for Plasmid from yeast let me know and I will post it, no kit needed, just too lazy to look for something that you probably already did 2.5 months ago!
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Old 06-18-2012, 06:45 AM
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Default Re: [Yeast] 2 micron plasmid extraction

learning
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extraction , micron , plasmid , yeast


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