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-   -   [Yeast] 2 micron plasmid extraction (http://www.molecularstation.com/forum/yeast-forum/46598-%5Byeast%5D-2-micron-plasmid-extraction.html)

Ellie Harrison 08-15-2008 11:25 AM

[Yeast] 2 micron plasmid extraction
 

Hi all,

I'm looking to extract naturally occurring 2 micron plasmid from yeast.
There seem to be a range of plasmid DNA preps but having tried the qiagen
plasmid kit and failed i would appreciate a recommendation before buying
into different kits.

Does anyone have any experience with this?

All help much appreciated

Ellie

PS. thanks for all replies regarding aAA selection- please feel free to
contact me if interested in the results!
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anubis2005 05-28-2011 06:40 AM

Re: [Yeast] 2 micron plasmid extraction
 
The Zymoprep™ Yeast Plasmid Miniprep II I heard is a very good kit.

pigfarmer 11-15-2011 08:23 PM

Re: [Yeast] 2 micron plasmid extraction
 
If you still do, or anyone else want an easy prep for Plasmid from yeast let me know and I will post it, no kit needed, just too lazy to look for something that you probably already did 2.5 months ago!

hairongxu88 06-18-2012 06:45 AM

Re: [Yeast] 2 micron plasmid extraction
 
learning


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