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Slow galactose induction

Slow galactose induction - Yeast Forum

Slow galactose induction - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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Old 09-04-2007, 05:54 PM
Kerry Geiler
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Default Slow galactose induction



Hi,

I am looking for suggestions as to why my galactose induction process
takes 6 hours. I am expressing CFP under a gal promoter in media with
no glucose and 2% galactose. I am getting fluorescence, but it takes
6 hours to see it. Any ideas why induction could be taking so long?

I grow the cells up in media containing no glucose (2%raffinose)
overnight. Then I induce 150uL of this culture with 2% galactose and
measure fluorescence every 15 minutes. I do not see fluorescence
distinguishable from background until about 6 hours later.

Thanks very much for any suggestions! Please reply to [Only registered users see links. ].
Thanks,
Kerry

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