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| Yeast Forum Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species. |
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#1
03-05-2007, 10:50 AM
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 Transformation problems I am having difficulties transforming an industrial strain of /_S. cerevisiae_/ by homologous recombination in URA3. I have tried the Gietz high-efficienty protocol and electroporation (Manivasakam and Schiestl, 1993). I am selecting for G418 (200ug/ml). None of the protocols had given me colonies. I am using approximately 5ug of a 5kb DNA fragment. Is this too much? Best regards Margarida  |
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