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[Yeast] FACS sorting/Regeneration of spheroplast

[Yeast] FACS sorting/Regeneration of spheroplast - Yeast Forum

[Yeast] FACS sorting/Regeneration of spheroplast - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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Old 01-30-2007, 10:27 PM
Wang, Xin-Xiang
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Default [Yeast] FACS sorting/Regeneration of spheroplast



Sure. But we are interested in something in the periplasmic space. We
seeminly cannot partially disrupt the cell wall while accessing the
interested product. We have to make spheroplast in order to detect it.
Thanks!

My question is: can the spheroplast (do delicate after removing the cell
wall) sustain the electro-charging during FACS sorting? I know you can
do flow cytometric testing with spheroplast, though. Any experience?

Xinxiang

-----Original Message-----
From: Eric Grote [mailto:[Only registered users see links. ]]
Sent: Tuesday, January 30, 2007 5:11 PM
To: Wang, Xin-Xiang
Subject: Re: [Yeast] FACS sorting/Regeneration of spheroplast


If you reduce the spheroplasting time, they will regenerate better.


On 1/29/07 12:45 PM, "Wang, Xin-Xiang" <[Only registered users see links. ]> wrote:

them in Top
sorting?


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facs , sorting or regeneration , spheroplast , yeast


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