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Yeast Forum Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


FACS sorting/Regeneration of spheroplast

FACS sorting/Regeneration of spheroplast - Yeast Forum

FACS sorting/Regeneration of spheroplast - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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Old 01-29-2007, 05:45 PM
Wang, Xin-Xiang
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Default FACS sorting/Regeneration of spheroplast



Hello,

Has anybody done FACS sorting with spheroplast and then regenerate them in Top agar? Would the charge in FACS machine burst the spheroplast during sorting? My regeneration rate was only 1%. Any hint to improve that? Thanks!

Xinxiang

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