Glycerol stocks from cultures in 2xYPAD (Anna Heidenblut)

Hi, Anna,

I truly don't see why it would be a problem. Actually, those cultures will
be growing very good and the cells will be quite robust. What I'd do is to
take the cells that you need for the transformation and place the rest in
the incubator while you are transforming, and, by the time you finish the
transformation, your cells will be probably nicely saturated and ready to
freeze (of course, with glycerol).

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Subject: Yeast Digest, Vol 20, Issue 4

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Today's Topics:

1. succinic semialdehyde determination (Juxiang Cao)
2. Glycerol stocks from cultures in 2xYPAD (Anna Heidenblut)
3. pombe ura4 library (Rich Maraia)


----------------------------------------------------------------------

Message: 1
Date: Thu, 18 Jan 2007 10:17:02 -0600
From: "Juxiang Cao" <[Only registered users see links. ]>
Subject: [Yeast] succinic semialdehyde determination
To: <[Only registered users see links. ].indiana.edu>
Message-ID: <[Only registered users see links. ]>
Content-Type: text/plain; charset=US-ASCII

Hi,
I would like to determine the succinic semialdehyde level from heat
stressed yeast cells by enzymatic assay, could I simply boil the cells
to prepare the crude cell extract? How is the stability of SSA?


Best regards
Juxiang Cao



------------------------------

Message: 2
Date: Fri, 19 Jan 2007 10:43:50 +0000
From: Anna Heidenblut <[Only registered users see links. ]>
Subject: [Yeast] Glycerol stocks from cultures in 2xYPAD
To: [Only registered users see links. ]
Message-ID: <[Only registered users see links. ]>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Dear all,

another elementary question:
Can it cause any problems when I prepare glycerol stocks from cultures
that were grown in double strength YPAD (YPD with additional adenin
added)? I use this 2xYPAD to grow pre-cultures for yeast transformation
and it would be very convenient to prepare glycerol stocks from the
pre-cultures immediately rather than setting up a separate culture in
YPD just for the preparation of glycerol stocks.

Any comments on that question are highly appreciated.

Anna

--
Dr. Anna Heidenblut (PhD)

Genome Integrity Group
Department of Molecular Oncology
Weatherall Institute of Molecular Medicine
Oxford University John Radcliffe Hospital,
Headley Way, Oxford, Oxfordshire OX3 9DS UK
Tel: 0044 / 1865 / 222419
[Only registered users see links. ]



------------------------------

Message: 3
Date: Fri, 19 Jan 2007 09:28:03 -0500
From: Rich Maraia <[Only registered users see links. ].gov>
Subject: [Yeast] pombe ura4 library
To: [Only registered users see links. ]
Message-ID: <p06230903c1d685c345ea@[137.187.221.10]>
Content-Type: text/plain; charset="us-ascii" ; format="flowed"

Does anyone have or know of a S. pombe genomic library on a
ura4+-containing plasmid?
--
Richard J. Maraia, M.D.
Captain, US Public Health Service, Commissioned Corps
Chief, Section on Molecular and Cell Biology
Genomics of Differentiation
National Institute of Child Health and Human Development
National institutes of Health
31 Center Drive,
Bld 31, Rm 2A25 MSC-2426
Bethesda, MD 20892-2426

Phone: 301 402-3567
Fax: 301 480-6863
[Only registered users see links. ]



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