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Glycerol stocks from cultures in 2xYPAD

Glycerol stocks from cultures in 2xYPAD - Yeast Forum

Glycerol stocks from cultures in 2xYPAD - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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Old 01-19-2007, 10:43 AM
Anna Heidenblut
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Default Glycerol stocks from cultures in 2xYPAD



Dear all,

another elementary question:
Can it cause any problems when I prepare glycerol stocks from cultures
that were grown in double strength YPAD (YPD with additional adenin
added)? I use this 2xYPAD to grow pre-cultures for yeast transformation
and it would be very convenient to prepare glycerol stocks from the
pre-cultures immediately rather than setting up a separate culture in
YPD just for the preparation of glycerol stocks.

Any comments on that question are highly appreciated.

Anna

--
Dr. Anna Heidenblut (PhD)

Genome Integrity Group
Department of Molecular Oncology
Weatherall Institute of Molecular Medicine
Oxford University John Radcliffe Hospital,
Headley Way, Oxford, Oxfordshire OX3 9DS UK
Tel: 0044 / 1865 / 222419
[Only registered users see links. ]

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