Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Yeast Forum
Register Search Today's Posts Mark Forums Read

Yeast Forum Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


separation of dead and live cells?

separation of dead and live cells? - Yeast Forum

separation of dead and live cells? - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 12-07-2006, 07:15 PM
Nicolas Brandes
Guest
 
Posts: n/a
Default separation of dead and live cells?





Hello,



I was wondering if anyone knows a method to separate dead and live yeast
cells BEFORE analyzing them with a fluorescence microscope.

Is it maybe possible to lyse the dead cells and then separate these lysed
cells from the live cells by centrifugation?

My aim is to get an enriched fraction of live cells without all the dead
cells (after ~ 10 days of growth I have a lot of dead cells in my solution I
want to get rid of)



Any ideas?







Thanks for help,

Nico





[Only registered users see links. ]









---------------------------

Nicolas Brandes
University of Michigan
Department of MCDB
Nat.Sci. Bldg., Room 4041 (Jakob lab)
830 N.University
Ann Arbor, MI 48109

Lab phone: +1-734-615-1957




Reply With Quote
Reply

Tags
cells , dead , live , separation


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
mouse hepatocytes isolation amicia Cell Biology and Cell Culture 39 05-11-2012 11:52 AM
On The Origins Of People, Mr. Obama Get Started, Now, Please! flexgonzaga@yahoo.com Botany Forum 3 10-19-2008 04:38 AM
Cell separation subhash juneja Basic Lab Protocols and Techniques 0 12-16-2007 07:04 PM
Politics And Cannibalism? Introducing The Dourties, Chelsea, Bill, Hillary, Barrack Obama, George Bush, Jr., And All Of Capital Hill! jon_johnfrancisayres@yahoo.com Microbiology Forum 0 10-06-2007 05:59 AM


All times are GMT. The time now is 08:07 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13643 seconds with 16 queries