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Problem with -His plates

Problem with -His plates - Yeast Forum

Problem with -His plates - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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Old 07-21-2006, 03:21 PM
Anna Heidenblut
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Default Problem with -His plates



Dear list,

I'm new to working with yeast and I've got trouble with my -His plates:

I've prepared to batches of -His plates using batches of CSM-His from
two different labs and adding glucose (Fisher Scientific), N-base
(ForMedium) and bacto-agar (BD). I've plated His- strains (which are
definitely OK) on these plates and they grow on both batches of plates.
I restreaked the yeast to the -His plates to make sure they don't carry
over too much His from the YPD they were grown in but they still grow
when restreaked on -His plates.

Has anybody ever experienced problems with His contamination of glucose,
N-base or bacto-agar?

Thank you for your help.

Anna

--
Dr. Anna Heidenblut (PhD)

Genome Integrity Group
Department of Molecular Oncology
Weatherall Institute of Molecular Medicine
Oxford University John Radcliffe Hospital,
Headley Way, Oxford, Oxfordshire OX3 9DS UK
Tel: 0044 / 1865 / 222419

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