I am an undergraduate student so my experience with yeast is limited.
I am looking for ways to delete or mutate cln1, cln2 and cln3 genes
from budding yeast for my project. I did some research and I found
site directed mutagenesis is a good option. But my time is limited. I
need to get it done about 3-4 months.
I would really appreciate if someone could give a time estimate of
doing site directed mutagenesis on these 3 genes or give me ideas
about which other methods I should look into. Or is it possible to
purchase such strains from the market?
I am really looking forward to hearing from you. Thank you.