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gene deletion/mutation in yeast

gene deletion/mutation in yeast - Yeast Forum

gene deletion/mutation in yeast - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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Old 05-24-2006, 12:01 PM
Mehmet Cetin
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Default gene deletion/mutation in yeast



Dear all,

I am an undergraduate student so my experience with yeast is limited.
I am looking for ways to delete or mutate cln1, cln2 and cln3 genes
from budding yeast for my project. I did some research and I found
site directed mutagenesis is a good option. But my time is limited. I
need to get it done about 3-4 months.

I would really appreciate if someone could give a time estimate of
doing site directed mutagenesis on these 3 genes or give me ideas
about which other methods I should look into. Or is it possible to
purchase such strains from the market?

I am really looking forward to hearing from you. Thank you.

Mehmet Cetin

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