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-   -   Cell Fractionation for Yeast (http://www.molecularstation.com/forum/yeast-forum/46330-cell-fractionation-yeast.html)

Audrey Brenot 10-07-2005 05:50 PM

Cell Fractionation for Yeast
 
Hi,
I am looking for a protocol or a kit to cell fractionate (cytosol, nucleus, membrane, cell
wall for example) yeast cells but I would like to assay the fractions for enzymatic
activity so I need to stay away from any denaturing conditions.
Could anybody help me?
Thanks.


Dr Audrey Brenot
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UCSF-Dpt of Cell and Tissue Biology
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