Cell Fractionation for Yeast
 

Hi,
I am looking for a protocol or a kit to cell fractionate (cytosol, nucleus, membrane, cell
wall for example) yeast cells but I would like to assay the fractions for enzymatic
activity so I need to stay away from any denaturing conditions.
Could anybody help me?
Thanks.


Dr Audrey Brenot
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UCSF-Dpt of Cell and Tissue Biology
521 Parnassus Ave (Room C742)
San Francisco, CA 94143
Tel: (415) 476-6850
Fax: (415) 476-0664