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heterologous expression in yeast

heterologous expression in yeast - Yeast Forum

heterologous expression in yeast - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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Old 11-30-2004, 01:13 AM
Dr. Pierre BERTHOMIEU
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Default heterologous expression in yeast



Hi,

We expressed an Arabidopsis cDNA in the wild-type W303 yeast strain
and obtained both the following two phenotypes:
- the cDNA expression INDUCED RESISTANCE to a toxic ionic
treatment in drop tests (on solid medium)
AND
- the cDNA expression INDUCED increased SENSITIVITY to the
same toxic ionic treatment in liquid culture.
We used the same culture medium, of course.
It might be of interested to know that the protein is supposed to be
secreted.

Has anybody already experienced such a strange situation ?
Any comment, advice or idea to try to understand this phenomenon
is welcome.

Yours

Dr. Pierre BERTHOMIEU
Assistant Professor
Biochimie et Physiologie Moleculaire des Plantes
UMR5004
Agro.M tel: (+33) 499612611
Place Viala fax: (+33) 467525737
34060 MONTPELLIER Cedex 1 e-mail: [Only registered users see links. ]
FRANCE

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