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background growth / uracil marker

background growth / uracil marker - Yeast Forum

background growth / uracil marker - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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Old 10-13-2004, 04:27 PM
Bruno.Dombrecht@csiro.au
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Default background growth / uracil marker



I've just started working on Y2H with Stratagene's Cytotrap system.
This system uses the cdc25H strain in combination with URA3 as
selection marker on the target-plasmid. However, I seem to get quite
some background growth of cdc25H on SD lacking uracil. Does anyone
have an explanation for this?


Cheers,

Bruno

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