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#1
10-13-2004, 04:27 PM
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 background growth / uracil marker I've just started working on Y2H with Stratagene's Cytotrap system. This system uses the cdc25H strain in combination with URA3 as selection marker on the target-plasmid. However, I seem to get quite some background growth of cdc25H on SD lacking uracil. Does anyone have an explanation for this? Cheers, Bruno ______________O_________oO_____________oO______o__ _____oO___________________ YEAST bionet newsgroup see: [Only registered users see links. ] YEAST e-mail: messages sent to [Only registered users see links. ] subscribe: mailto:[Only registered users see links. ].net and say: subscribe yeast unsubscribe: mailto:[Only registered users see links. ].net and say: unsubscribe yeast YEAST on the WWW: [Only registered users see links. ] Newsgroup Moderator SGD Curators: [Only registered users see links. ]  |
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| background , growth , marker , uracil |
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