requesting information on yeast promoter less vector pAS 201

Hi Franco,

While it is true that many of the original promoter bashing
experiments were done on plasmid borne promoter-reporter cassettes it
is clear that the chromosomal context of the reporter plays an
important role in gene regulation and should not be ignored. A good
example of this effect can be seen with the MET3 promoter which is
tightly regulated in its native context (ie in its chromosomal
location) but becomes leaky when placed in a plasmid (either ARS-CEN
or 2 micron). There are likely multiple factors that lead to this
affect, histone organisation differences, repressive flanking
sequences, disturbance of the promoter/regulator balance (2micron
plasmids present 50-100 copies of the promoter!!!) etc....


Cheers

Martin


On 9/23/03 9:30, "francov" <[Only registered users see links. ].med.ualberta.ca> wrote:


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