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please tell me what is the shortest active fragment of pgk

please tell me what is the shortest active fragment of pgk - Yeast Forum

please tell me what is the shortest active fragment of pgk - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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Old 07-04-2003, 04:45 AM
[gb2312] shude yang
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Default please tell me what is the shortest active fragment of pgk



Hello:

I have spent half a year to modify a expression vector
of yeast. A important part of work was to insert a
promoter of phosphoglycerate kinase (PGK) and a PGK
terminator into pFL39.However, after having inserted a
630bp- length fragment of PGK promoter drevied from
another vector, I found that there were various kind
of PGK promoters with different length in database,
such as of 1484bp, 1496bp, 603bp, 400bp(it is called
active site of PGK promoter) fragment. Therefore, I am
anxiously worried about whether the PGK promoter of
603bp which I used in my work is active enough to
initiate transcription. As knowing little about the
characteristics of promoter structure, I hope you can
give a urgent help. The sequence of PGK promoter I
used is showed as follows:



CCCAAGCTTACCTGCTGCGCATTGTTTTATATTTGTTGTAAAAAGTAGAT AATTACTTCCTTGATGATCTGTAAAAAAGAGAAAAAGAAAGCATCTAAGA ACTTGAAAAACTACGAATTAGAAAAGACCAAATATGTATTTCTTGCATTG ACCAATTTATGCAAGTTTATATATATGTAAATGTAAGTTTCACGAGGTTC TACTAAACTAAACCACCCCCTTGGTTAGAAGAAAAGAGTGTGTGAGAACA GGCTGTTGTTGTCACACGATTCGGACAATTCTGTTTGAAAGAGAGAGAGT AACAGTACGATCGAACGAACTTTGCTCTGGAGATCACAGTGGGCATCATA GCATGTGGTACTAAACCCTTTCCCGCCATTCCAGAACCTTCGATTGCTTG TTACAAAACCTGTGAGCCGTCGCTAGGACCTTGTTGTGTGACGAAATTGG AAGCTGCAATCAATAGGAAGACAGGAAGTCGAGCGTGTCTGGGTTTTTTC AGTTTTGTTCTTTTTGCAAACAAATCACGAGCGACGGTAATTTCTTTCTC GATAAGAGGCCACGTGCTTTATGAGGGTAACATCAATTCAAGATCTGAAT TCC



Thank you very much

Shude Yang
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