I'm new to this forum and I just have a question for you all.. if someone could be nice enough to answer me.. i am having some problems
I just started working on a yeast two hybrid project using the LexA system. My problem begins here: First I froze the co-transformed yeasts (i.e. with bait and library) in TE buffer/Glycerol with MgSO4 and then I titrated. At 1:10 to 1:10000 dilutions there are lots of yeast cells (microscope confirmed that smear had yeast). My problem is that at a 100,000 dilution (next 10-fold dilution), there is no yeast growth AT ALL.
Please someone tell me why there is lots of growth in the more concentrated one than at the diluted sample? I am a LOST student...