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|Yeast Forum Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.|
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I've read many protocols about trasformation in pichia pastoris. However i didn't to stabilish one that give me good results. In my electroporation i've never had transformants colonies and when i achieve some result in my electroporation i have colonies in my negative control (electroporation with water).
Somebody can help me? some suggestions about it?
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