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Yeast Forum Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


Transformation

Transformation - Yeast Forum

Transformation - Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.


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  #1  
Old 10-20-2008, 03:45 PM
Pipette Filler
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Default Transformation



I've read many protocols about trasformation in pichia pastoris. However i didn't to stabilish one that give me good results. In my electroporation i've never had transformants colonies and when i achieve some result in my electroporation i have colonies in my negative control (electroporation with water).
Somebody can help me? some suggestions about it?
Thx
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Old 06-18-2012, 06:59 AM
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Default Re: Transformation

read more paper to find the solution
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