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Western Blot Forum

Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

Western Blot Forum

Western Blot Forum - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

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buffer, lysis, needed, pellet, ripa, urgent, viscous

Sticky: Lysis for WB with Ripa Buffer - too viscous, no pellet - urgent help needed, please (

1 2 )

Anne-Marie

02-01-2013 05:03 PM
by chandra.S.K.

30,892

Sticky: Western Blot Optimization (

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dave2006

04-29-2011 09:15 AM
by sasha_028

10,521

blotting, semi-dry, semidry, western blot

Sticky: Semi-dry blotting (

1 2 )

sniftyhit13

03-25-2011 12:10 AM
by danfive

4,898

amount, blot, calculate, gel, load, protein, western

Sticky: How to calculate amount of protein to load into Western Blot gel

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03-15-2011 09:53 PM
by TorontoStudent

15,266

blotting, phosphorylated, proteins, western

Sticky: Western Blotting for Phosphorylated Proteins (

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sciencemandan

02-20-2011 12:38 PM
by gehrs

6,440

blot, control, protein, secreted, western

Sticky: Western blot secreted protein control

alphabetty

02-20-2011 12:33 PM
by gehrs

5,001

bands, disappears, phospho, problem, quickly, signal, western

Sticky: Problem: phospho signal quickly disappears

amei

02-08-2011 02:04 PM
by Cancer Biologist

1,217

Sticky: Loading Control

montMolec

11-20-2010 01:14 AM
by jasonbourne1

11,822

Anybody knows what is the protein(s)? Thanks.

xieche

09-14-2011 01:14 PM
by xieche

303

Immunoprecipitated samples

pmitra

09-13-2011 05:44 PM
by pmitra

599

blocking, blocking buffer, buffer, licor, precipitation, yellow

Yellow precipitation in Licor Blocking buffer

histonerunner

09-10-2011 02:19 PM
by Ana_Lonic

712

Western blot with more strong bands

biotom

09-08-2011 01:49 PM
by biotom

593

blue, blue native gel, coomassie blue, gels, native, problem, transfer, western blot

Transfer problem from Blue Native Gels

Postdoc 2011

08-31-2011 07:24 PM
by malladip

1,875

Ladder problem

NeuroLabChick

08-21-2011 01:15 AM
by NeuroLabChick

433

Can't find PARP in WB

DMPereira

08-20-2011 07:14 PM
by sedrais

415

HRP-conjugated antibody as primary antibody?

Chunfang

08-15-2011 03:01 AM
by Chunfang

777

antibodies, incubate, membrane, multiple, primary, round

Incubate membrane with multiple primary antibodies in same round?

Emily.Koury

08-04-2011 04:11 PM
by Emily.Koury

624

How Can I Control My Loading?

Rahi

07-25-2011 04:42 AM
by admin

436

Loading control issues

Bev

06-30-2011 08:02 AM
by Bev

518

protein preparation

bellonit

06-01-2011 09:35 PM
by bellonit

479

HIF1A bands not appearing

thebadman

06-01-2011 06:07 AM
by thebadman

479

actin, bands, betatubulin, conditions, double, nonreducing, total

double total actin and beta-tubulin bands under non-reducing conditions???

Dani007

05-23-2011 05:21 PM
by kdude

1,752

Protein not migrating down gel

J3nny

05-20-2011 04:22 PM
by kmmahan

630

Cytochrome P450 (

1 2 )

nawi

05-13-2011 07:40 AM
by nawi

1,432

Loading problems - please help

kforema

05-12-2011 10:53 AM
by kforema

232

Loading problems - please help

kforema

05-12-2011 10:47 AM
by kforema

255

Laemmli Buffer

nawi

05-09-2011 05:17 PM
by danfive

949

Western Blot Stoping PointS!! ADD YOURS

mai-mb

05-04-2011 05:45 PM
by mai-mb

1,973

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Western Blot Forum

Western Blot Forum - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.