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Western Blot Forum

Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

Western Blot Forum

Western Blot Forum - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

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buffer, lysis, needed, pellet, ripa, urgent, viscous

Sticky: Lysis for WB with Ripa Buffer - too viscous, no pellet - urgent help needed, please (

1 2 )

Anne-Marie

02-01-2013 05:03 PM
by chandra.S.K.

31,259

Sticky: Western Blot Optimization (

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04-29-2011 09:15 AM
by sasha_028

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blotting, semi-dry, semidry, western blot

Sticky: Semi-dry blotting (

1 2 )

sniftyhit13

03-25-2011 12:10 AM
by danfive

4,998

amount, blot, calculate, gel, load, protein, western

Sticky: How to calculate amount of protein to load into Western Blot gel

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03-15-2011 09:53 PM
by TorontoStudent

15,724

blotting, phosphorylated, proteins, western

Sticky: Western Blotting for Phosphorylated Proteins (

1 2 )

sciencemandan

02-20-2011 12:38 PM
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blot, control, protein, secreted, western

Sticky: Western blot secreted protein control

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bands, disappears, phospho, problem, quickly, signal, western

Sticky: Problem: phospho signal quickly disappears

amei

02-08-2011 02:04 PM
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Sticky: Loading Control

montMolec

11-20-2010 01:14 AM
by jasonbourne1

11,878

Storing PVDF membrane for more than 3 weeks

cplee

05-08-2012 12:10 AM
by cplee

440

Western Blot - Vertical Streaking

ashhbel

04-18-2012 02:21 PM
by ashhbel

462

Dark background on blots

raghu.dvs

03-16-2012 01:29 PM
by lendaSoL

503

SERCA2a: various bands

sammieC

03-16-2012 01:05 PM
by Jesi

719

Protein Extraction from different cells organs

rizky

02-24-2012 02:53 AM
by admin

520

blotting, content, extracted, high, lipid, organs, protein, tissue, western

Western Blotting from Protein Extracted from Organs Tissue with High Lipid Content

rizky

02-24-2012 02:45 AM
by admin

498

Removing Albumin from Samples

snlee06

02-23-2012 10:22 AM
by Zagami

627

Membrane Incubation Plates/Trays

sciencegirl11

02-16-2012 05:30 PM
by JuanM

862

Commercial western-blot membranes

marnedo

02-16-2012 09:08 AM
by Zagami

472

$controls, fraction, loading, membrane$ Loading Controls for Membrane Fraction

CPF

02-15-2012 03:28 AM
by admin

859

analysis, calculations, control, issues, loading, loading control, normalization, western blot

Loading control Issues and Normalization

br.gruenewald

01-04-2012 11:52 AM
by obama

933

blot, stopped, suddenly, western, working

Western Blot suddenly stopped working!!! :(

biotoxgal

12-31-2011 10:47 PM
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858

Loading problems - please help

kforema

12-31-2011 10:39 PM
by lyrachang

410

blot, gel, increasing, side, signal, trend, western

Western Blot Gel Trend Increasing Signal from One Side to Other?

preethi

12-31-2011 10:30 PM
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330

Uneven transfer across blot

mcorso10

12-31-2011 10:28 PM
by lyrachang

578

iBlot Nitrocellulose membrane dried!

fmhakami

12-21-2011 07:40 PM
by obama

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high, nmda, nmda western, receptor, signal, weights, western

NMDA receptor western with signal at high weights

Horrible Clarity

12-15-2011 09:37 PM
by Horrible Clarity

453

membrane, nitrocellulose, protein, transfer

Can I use the same nitrocellulose membrane twice for protein transfer?

hhzheng

12-14-2011 05:21 AM
by raghu.dvs

427

length, protein, relationship, size, transfer, voltage, whatis

whatis the relationship among voltage, protein size, and length of transfer?

William liu

11-14-2011 05:57 AM
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727

blood, cell, control, loading, proteins, red, register

Loading control that does not register red blood cell proteins

synthesize

10-04-2011 06:03 PM
by synthesize

1,305

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Western Blot Forum

Western Blot Forum - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.