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Western Blot Forum

Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

Western Blot Forum

Western Blot Forum - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

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blotting, phosphorylated, proteins, western

Sticky: Western Blotting for Phosphorylated Proteins (

1 2 )

sciencemandan

02-20-2011 12:38 PM
by gehrs

6,413

Sticky: Western Blot Optimization (

1 2 )

dave2006

04-29-2011 09:15 AM
by sasha_028

10,506

buffer, lysis, needed, pellet, ripa, urgent, viscous

Sticky: Lysis for WB with Ripa Buffer - too viscous, no pellet - urgent help needed, please (

1 2 )

Anne-Marie

02-01-2013 05:03 PM
by chandra.S.K.

30,819

blotting, semi-dry, semidry, western blot

Sticky: Semi-dry blotting (

1 2 )

sniftyhit13

03-25-2011 12:10 AM
by danfive

4,876

Sticky: Loading Control

montMolec

11-20-2010 01:14 AM
by jasonbourne1

11,804

amount, blot, calculate, gel, load, protein, western

Sticky: How to calculate amount of protein to load into Western Blot gel

wendychang

03-15-2011 09:53 PM
by TorontoStudent

15,167

blot, control, protein, secreted, western

Sticky: Western blot secreted protein control

alphabetty

02-20-2011 12:33 PM
by gehrs

4,989

bands, disappears, phospho, problem, quickly, signal, western

Sticky: Problem: phospho signal quickly disappears

amei

02-08-2011 02:04 PM
by Cancer Biologist

1,213

background, blot, troubleshooting, western

Western Blot Background Troubleshooting (

1 2 )

molecule2005

10-07-2010 11:49 AM
by meenurohini

15,249

transfer time (

1 2 )

nadia naeem

08-26-2008 06:18 AM
by nadia naeem

6,006

Membrane Storing (

1 2 )

Arastho

07-30-2009 04:03 PM
by Mad Scientist

4,564

Transfer Issues to PVDF (

1 2 )

Kaitou Kid

04-07-2009 07:32 AM
by biohou

8,049

blotting, extractions, nuclear, protein, tissue

Blotting a nuclear protein and nuclear protein extractions in tissue (

1 2 )

Pete_2007

02-08-2008 08:31 PM
by danfive

4,797

antibody, blot, reusing, western, western blot

Reusing Antibody After Western Blot (

1 2 )

gandalfthegrey

02-13-2010 07:31 PM
by Tfal

6,689

IP washing step (

1 2 )

david1259

12-11-2007 10:32 PM
by danfive

4,147

No membrane receptor band at all (

1 2 )

t.christides

06-30-2010 08:14 PM
by danfive

1,062

Cytochrome P450 (

1 2 )

nawi

05-13-2011 07:40 AM
by nawi

1,430

controlto, loading, loading control, stripping, western blot, work

Can't get loading controlto work after stripping

psingh

08-27-2008 11:19 PM
by psingh

1,595

Help with blurry bands!

breathoffire

07-03-2010 04:46 AM
by danfive

1,917

Removing Albumin from Samples

snlee06

02-23-2012 10:22 AM
by Zagami

606

multiple, multiple gels, preparing, sample, sample prep, western blot, westerns

Preparing enough sample for multiple westerns

jeeep

06-26-2012 06:41 AM
by butters

407

Novel(?) Western Blotting question

fitbrit

09-18-2007 08:09 PM
by fitbrit

1,377

Storage of PVDF membranes after western

cintiadp

10-10-2007 04:05 PM
by danfive

11,360

equal loading on western blot?

kstovall

07-11-2009 05:51 AM
by Anastasia_S

5,211

blotchy, irregular bands

rufus

09-29-2009 06:17 PM
by Tiffy

1,543

serum sample.....

sara.bi

10-13-2010 01:11 PM
by sara.bi

1,561

Split bands, weak signal in the middle of all bands

brainStorm

12-23-2010 02:51 PM
by admin

1,055

Loading control for serum samples?

Allison M

04-17-2009 05:50 PM
by Jordan

3,315

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Western Blot Forum

Western Blot Forum - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.