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Western Blot Forum

Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

Western Blot Forum

Western Blot Forum - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

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amount, blot, calculate, gel, load, protein, western

Sticky: How to calculate amount of protein to load into Western Blot gel

wendychang

03-15-2011 09:53 PM
by TorontoStudent

15,241

Sticky: Loading Control

montMolec

11-20-2010 01:14 AM
by jasonbourne1

11,814

buffer, lysis, needed, pellet, ripa, urgent, viscous

Sticky: Lysis for WB with Ripa Buffer - too viscous, no pellet - urgent help needed, please (

1 2 )

Anne-Marie

02-01-2013 05:03 PM
by chandra.S.K.

30,869

bands, disappears, phospho, problem, quickly, signal, western

Sticky: Problem: phospho signal quickly disappears

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02-08-2011 02:04 PM
by Cancer Biologist

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blotting, semi-dry, semidry, western blot

Sticky: Semi-dry blotting (

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03-25-2011 12:10 AM
by danfive

4,891

Sticky: Western Blot Optimization (

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dave2006

04-29-2011 09:15 AM
by sasha_028

10,518

blot, control, protein, secreted, western

Sticky: Western blot secreted protein control

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02-20-2011 12:33 PM
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blotting, phosphorylated, proteins, western

Sticky: Western Blotting for Phosphorylated Proteins (

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02-20-2011 12:38 PM
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6,430

blots, center effect, detection trouble, large protein, western, western blot

"Center effect" in Western Blots

Nattylad

11-01-2010 07:54 PM
by fatrat

536

"house keeping"??

HTS76

12-08-2009 08:30 PM
by danfive

485

1 gel, 2 probes

AdmiralJose

02-27-2008 07:04 PM
by AdmiralJose

542

11KD detection

terestivo

07-05-2007 01:08 AM
by moleculardude

1,022

a different way to develop

Andre212

07-24-2009 12:07 PM
by Andre212

279

Absence of Bands on Western Blot Film

maprinzz

09-12-2008 04:26 PM
by celina

1,676

air bubble while loading sample.

IoDiNE

10-19-2007 09:20 AM
by Doc

1,146

alpha 1 a

Amberbl

11-06-2009 05:37 PM
by Amberbl

456

alpha tubulin vs beta tubulin?

RadSyentist

09-21-2011 08:36 PM
by RadSyentist

516

alternate way for quantification ?

bpk2006

06-13-2009 10:13 AM
by bpk2006

283

anti-GFP antibody

MicroScope

08-06-2009 07:58 PM
by MicroScope

1,503

Antibody dilution

russell0409

08-20-2008 05:53 PM
by liono

2,030

antibody incubation

yruano

08-13-2012 11:39 AM
by yruano

313

Antibody raised in mouse used on mouse tissue?

TorontoStudent

04-16-2010 01:46 PM
by Ileana

1,384

Antibody Reuse for Western Blots?

IoDiNE

08-26-2008 10:24 PM
by proatpcr

989

antibody works in IHC but not on blot?

tyronegenade

08-03-2009 09:01 AM
by tyronegenade

1,210

Antybodies for caspases??

Agustin

04-22-2009 04:46 AM
by Agustin

268

Anybody knows what is the protein(s)? Thanks.

xieche

09-14-2011 01:14 PM
by xieche

302

cell, lysates, protein preparation, rnase or dnase, treating, viscous

Anyone treating Cell lysates with RNAse/DNAse

danfive

05-15-2008 06:22 PM
by admin

1,279

alkaline phosphatase, hrp, secondary, signal, western

Poll: AP vs. HRP secondary

sarahstudeman

06-29-2009 09:08 PM
by Bonnie

3,349

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Western Blot Forum

Western Blot Forum - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.