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Western Blot Forum Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


Salt effect on SDS page

Salt effect on SDS page - Western Blot Forum

Salt effect on SDS page - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.


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Old 06-14-2013, 11:03 PM
Pipette Filler
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Default Salt effect on SDS page



I heard that salt concentration can affect the size a protein runs on SDS page. Is this true? And also, is it usually bigger or smaller or no way to tell?

This is a 4-12% Bis-Tris gel and its stained with coomassie.

Thanks
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